Effect of Bioaugmentation with Anaerobic Fungi Isolated from Ruminants on the Hydrolysis of Corn Silage and Phragmites australis

Anaerobic fungi produce extracellular hydrolytic enzymes that facilitate degradation of cellulose and hemicellulose in ruminants. The purpose this work was to study the impact three different anaerobic fungal species (Anaeromyces mucronatus YE505, Neocallimastix frontalis 27, Piromyces rhizinflatus YM600) on hydrolysis two lignocellulosic substrates, corn (Zea mays L.) silage reed (Phragmites australis (Cav.) Trin. ex Steud.). Biomass from each plant incubated anaerobically for 11 days either presence live inoculum or with heat-inactivated (control) inoculum. Headspace gas composition, dry matter loss, soluble chemical oxygen demand, concentration volatile fatty acids, composition were measured before after hydrolysis. While some microbial activity observed, inoculation did not result any significant difference type biomass tested, likely due low survival under experimental conditions tested. premise utilizing unique biological activities biotechnology applications remains promising, further research optimizing culturing process is necessary.